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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha : |
25/01/2022 |
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Actualizado : |
25/01/2022 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
LOUGE URIARTE, E.; GONZÁLEZ PASAYO, R.; MASSÓ, M.; CARRERA PAÉZ, L.; DOMÍNGUEZ MONCLA, M.; DONIS, N.; MALENA, R.; MÉNDEZ, A.; MORRELL, E.; GIANNITTI, F.; ARMENDANO, J.I.; FAVERIN, C.; CENTRÓN, D.; PARREÑO, V.; ODEÓN, A.C.; QUIROGA, M.P.; MOREIRA, A.R. |
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Afiliación : |
ENRIQUE L. LOUGE URIARTE, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; RAMÓN A. GOZÁLEZ PASAYO, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; MARIANA MASSÓ, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; LAURA CARRERA PAÉZ, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; MANUEL DOMÍNGUEZ MONCLA, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; NICOLÁS DONIS, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; ROSANA MALENA, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; ALEJANDRA MÉNDEZ, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; ELEONORA MORRELL, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; FEDERICO GIANNITTI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; JOAQUÍN I. ARMENDANO, Departamento de Fisiopatología, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires, Paraje Arroyo Seco s/n, Tandil, 7000, Argentina; CLAUDIA FAVERIN, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; DANIELA CENTRÓN, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; VIVIANA PARREÑO, Incuinta, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Virología e Innovaciones Tecnológicas, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IVIT, INTA-CONICET), Castelar, Buenos Aires, 1712, Argentina; ANSELMO C. ODEÓN, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina; MARÍA PAULA QUIROGA, Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, C1121ABG, Argentina; ANA RITA MOREIRA, Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Balcarce, Buenos Aires, Argentina. |
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Título : |
Molecular characterization of multidrug-resistant Escherichia coli of the phylogroups A and C in dairy calves with meningitis and septicemia. |
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Fecha de publicación : |
2022 |
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Fuente / Imprenta : |
Microbial Pathogenesis, 2022, Volume 163, Article number 105378. doi: https://doi.org/10.1016/j.micpath.2021.105378 |
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ISSN : |
0882-4010 |
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DOI : |
10.1016/j.micpath.2021.105378 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 8 October 2021; Received in revised form 21 December 2021; Accepted 28 December 2021; Available online 1 January 2022.
Corresponding authors: Louge Uriarte, E.L.; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Ruta 226 km 73.5, Balcarce, Buenos Aires, Argentina; email:lougeuriarte.enrique@inta.gob.ar --- Quiroga, M.P.; Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina; email:paula.quiroga@conicet.gov.ar -- |
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Contenido : |
ABSTRACT.- Escherichia coli is an important cause of septicemia (SEPEC) and neonatal meningitis (NMEC) in dairy calves. However, the diversity of virulence profiles, phylogroups, antimicrobial resistance patterns, carriage of integron structures, and fluoroquinolone (FQ) resistance mechanisms have not been fully investigated. Also, there is a paucity of knowledge about the virulence profiles and frequency of potential SEPEC in feces from calves with or without diarrhea. This study aimed to characterize the virulence potential, phylogroups, antimicrobial susceptibility, integron content, and FQ-resistance mechanisms in Escherichia coli isolated from calves with meningitis and septicemia. Additionally, the virulence genes (VGs) and profiles of E. coli isolated from diarrheic and non-diarrheic calves were compared between them and together with NMEC and SEPEC in order to identify shared profiles. Tissue and fluid samples from eight dairy calves with septicemia, four of which had concurrent meningitis, were processed for bacteriology and histopathology. Typing of VGs was assessed in 166 isolates from diverse samples of each calf. Selected isolates were evaluated for antimicrobial susceptibility by the disk diffusion test. Phylogroups, integron gene cassettes cartography, and FQ-resistance determinants were analyzed by PCR, sequencing, and bioinformatic tools. Furthermore, 109 fecal samples and 700 fecal isolates from dairy calves with or without diarrhea were evaluated to detect 19 VGs by uniplex PCR. Highly diverse VG profiles were characterized among NMEC and SEPEC isolates, but iucD was the predominant virulence marker. Histologic lesions in all calves supported their pathogenicity. Selected isolates mainly belonged to phylogroups A and C and showed multidrug resistance. Classic (dfrA17 and arr3-dfrA27) and complex (dfrA17-aadA5::ISCR1::blaCTX-M-2) class 1 integrons were identified. Target-site mutations in GyrA (S83L and D87N) and ParC (S80I) encoding genes were associated with FQ resistance. The VGs detected more frequently in fecal samples included f17G (50%), papC (30%), iucD (20%), clpG (19%), eae (16%), and afaE-8 (13%). Fecal isolates displaying the profiles of f17 or potential SEPEC were found in 25% of calves with and without diarrhea. The frequency of E. coli VGs and profiles did not differ between both groups (p > 0.05) and were identical or similar to those found in NMEC and SEPEC. Overall, multidrug-resistant E. coli isolates with diverse VG profiles and belonging to phylogroups A and C can be implicated in natural cases of meningitis and septicemia. Their resistance phenotypes can be partially explained by class 1 integron gene cassettes and target-site mutations in gyrA and parC. These results highlight the value of antimicrobial resistance surveillance in pathogenic bacteria isolated from food-producing animals. Besides, calves frequently shed potential SEPEC in their feces as commensals (?Trojan horse?). Thus, these bacteria may be disseminated in the farm environment, causing septicemia and meningitis under predisposing factors.
© 2022 Elsevier Ltd MenosABSTRACT.- Escherichia coli is an important cause of septicemia (SEPEC) and neonatal meningitis (NMEC) in dairy calves. However, the diversity of virulence profiles, phylogroups, antimicrobial resistance patterns, carriage of integron structures, and fluoroquinolone (FQ) resistance mechanisms have not been fully investigated. Also, there is a paucity of knowledge about the virulence profiles and frequency of potential SEPEC in feces from calves with or without diarrhea. This study aimed to characterize the virulence potential, phylogroups, antimicrobial susceptibility, integron content, and FQ-resistance mechanisms in Escherichia coli isolated from calves with meningitis and septicemia. Additionally, the virulence genes (VGs) and profiles of E. coli isolated from diarrheic and non-diarrheic calves were compared between them and together with NMEC and SEPEC in order to identify shared profiles. Tissue and fluid samples from eight dairy calves with septicemia, four of which had concurrent meningitis, were processed for bacteriology and histopathology. Typing of VGs was assessed in 166 isolates from diverse samples of each calf. Selected isolates were evaluated for antimicrobial susceptibility by the disk diffusion test. Phylogroups, integron gene cassettes cartography, and FQ-resistance determinants were analyzed by PCR, sequencing, and bioinformatic tools. Furthermore, 109 fecal samples and 700 fecal isolates from dairy calves with or without diarrhea were evaluated to detect... Presentar Todo |
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Palabras claves : |
CALVES; E. COLI; Integrons; Multidrug resistance; Phylogroups; Quinolone resistance-determining region (QRDR) mutations; Virulence genes. |
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Asunto categoría : |
L10 Genética y mejoramiento animal |
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Marc : |
LEADER 05268naa a2200433 a 4500
001 1062698
005 2022-01-25
008 2022 bl uuuu u00u1 u #d
022 $a0882-4010
024 7 $a10.1016/j.micpath.2021.105378$2DOI
100 1 $aLOUGE URIARTE, E.
245 $aMolecular characterization of multidrug-resistant Escherichia coli of the phylogroups A and C in dairy calves with meningitis and septicemia.$h[electronic resource]
260 $c2022
500 $aArticle history: Received 8 October 2021; Received in revised form 21 December 2021; Accepted 28 December 2021; Available online 1 January 2022. Corresponding authors: Louge Uriarte, E.L.; Instituto Nacional de Tecnología Agropecuaria (INTA), Instituto de Innovación para la Producción Agropecuaria y Desarrollo Sostenible, INTA-Consejo Nacional de Investigaciones Científicas y Técnicas (IPADS, INTA-CONICET), Ruta 226 km 73.5, Balcarce, Buenos Aires, Argentina; email:lougeuriarte.enrique@inta.gob.ar --- Quiroga, M.P.; Instituto de Investigaciones en Microbiología y Parasitología Médica, Facultad de Medicina, Universidad de Buenos Aires-Consejo Nacional de Investigaciones Científicas y Técnicas (IMPaM, UBA-CONICET), Ciudad Autónoma de Buenos Aires, Argentina; email:paula.quiroga@conicet.gov.ar --
520 $aABSTRACT.- Escherichia coli is an important cause of septicemia (SEPEC) and neonatal meningitis (NMEC) in dairy calves. However, the diversity of virulence profiles, phylogroups, antimicrobial resistance patterns, carriage of integron structures, and fluoroquinolone (FQ) resistance mechanisms have not been fully investigated. Also, there is a paucity of knowledge about the virulence profiles and frequency of potential SEPEC in feces from calves with or without diarrhea. This study aimed to characterize the virulence potential, phylogroups, antimicrobial susceptibility, integron content, and FQ-resistance mechanisms in Escherichia coli isolated from calves with meningitis and septicemia. Additionally, the virulence genes (VGs) and profiles of E. coli isolated from diarrheic and non-diarrheic calves were compared between them and together with NMEC and SEPEC in order to identify shared profiles. Tissue and fluid samples from eight dairy calves with septicemia, four of which had concurrent meningitis, were processed for bacteriology and histopathology. Typing of VGs was assessed in 166 isolates from diverse samples of each calf. Selected isolates were evaluated for antimicrobial susceptibility by the disk diffusion test. Phylogroups, integron gene cassettes cartography, and FQ-resistance determinants were analyzed by PCR, sequencing, and bioinformatic tools. Furthermore, 109 fecal samples and 700 fecal isolates from dairy calves with or without diarrhea were evaluated to detect 19 VGs by uniplex PCR. Highly diverse VG profiles were characterized among NMEC and SEPEC isolates, but iucD was the predominant virulence marker. Histologic lesions in all calves supported their pathogenicity. Selected isolates mainly belonged to phylogroups A and C and showed multidrug resistance. Classic (dfrA17 and arr3-dfrA27) and complex (dfrA17-aadA5::ISCR1::blaCTX-M-2) class 1 integrons were identified. Target-site mutations in GyrA (S83L and D87N) and ParC (S80I) encoding genes were associated with FQ resistance. The VGs detected more frequently in fecal samples included f17G (50%), papC (30%), iucD (20%), clpG (19%), eae (16%), and afaE-8 (13%). Fecal isolates displaying the profiles of f17 or potential SEPEC were found in 25% of calves with and without diarrhea. The frequency of E. coli VGs and profiles did not differ between both groups (p > 0.05) and were identical or similar to those found in NMEC and SEPEC. Overall, multidrug-resistant E. coli isolates with diverse VG profiles and belonging to phylogroups A and C can be implicated in natural cases of meningitis and septicemia. Their resistance phenotypes can be partially explained by class 1 integron gene cassettes and target-site mutations in gyrA and parC. These results highlight the value of antimicrobial resistance surveillance in pathogenic bacteria isolated from food-producing animals. Besides, calves frequently shed potential SEPEC in their feces as commensals (?Trojan horse?). Thus, these bacteria may be disseminated in the farm environment, causing septicemia and meningitis under predisposing factors. © 2022 Elsevier Ltd
653 $aCALVES
653 $aE. COLI
653 $aIntegrons
653 $aMultidrug resistance
653 $aPhylogroups
653 $aQuinolone resistance-determining region (QRDR) mutations
653 $aVirulence genes
700 1 $aGONZÁLEZ PASAYO, R.
700 1 $aMASSÓ, M.
700 1 $aCARRERA PAÉZ, L.
700 1 $aDOMÍNGUEZ MONCLA, M.
700 1 $aDONIS, N.
700 1 $aMALENA, R.
700 1 $aMÉNDEZ, A.
700 1 $aMORRELL, E.
700 1 $aGIANNITTI, F.
700 1 $aARMENDANO, J.I.
700 1 $aFAVERIN, C.
700 1 $aCENTRÓN, D.
700 1 $aPARREÑO, V.
700 1 $aODEÓN, A.C.
700 1 $aQUIROGA, M.P.
700 1 $aMOREIRA, A.R.
773 $tMicrobial Pathogenesis, 2022, Volume 163, Article number 105378. doi: https://doi.org/10.1016/j.micpath.2021.105378
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INIA Las Brujas (LB) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
19/04/2023 |
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Actualizado : |
26/01/2024 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
CAZZULI, F.; BREMM, C.; JAURENA, M.; POPPI, D.; DURANTE, M.; BENVENUTTI, M.A.; SAVIAN, J.V.; DEVINCENZI, T.; ROVIRA, P.J.; LAGOMARSINO, X.; HIRIGOYEN, A.; LATTANZI, F. |
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Afiliación : |
FIORELLA CARLA CAZZULI ALBA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; CAROLINA BREMM, Grazing Ecology Research Group, Federal University of Rio Grande do Sul, Porto, Alegre, Brazil; MARTIN ALEJANDRO JAURENA BARRIOS, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; DENNIS POPPI, School of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD, Australia; MARTÍN DURANTE, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Concepción del Uruguay, Concepción del Uruguay, Argentina; MARCELO ADOLFO BENVENUTTI, Department of Agriculture and Fisheries, The University of Queensland Gatton campus, Gatton, QLD, Australia; JEAN VICTOR SAVIAN, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; THAIS DEVINCENZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; XIMENA MARIA LAGOMARSINO LARRIERA, FCA-UDE, Facultad de Ciencias Agrarias, Universidad de la Empresa, Luis Alberto de Herrera, Montevideo, 2890, Uruguay; ANDRES EDUARDO HIRIGOYEN DOMINGUEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO A. LATTANZI, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
The defoliation dynamics of a stockpiled native grassland pasture follow similar patterns between supplemented and unsupplemented beef calves. |
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Fecha de publicación : |
2023 |
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Fuente / Imprenta : |
Grass and Forage Science, 2023, Vol.78, Issue 2, pages 306-316. doi: https://doi.org/10.1111/gfs.12608 |
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ISSN : |
0142-5242 |
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DOI : |
10.1111/gfs.12608 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 24 November 2022; Revised 14 March 2023; Accepted 15 March 2023. -- Correspondence author: Cazzuli, F.; Instituto Nacional de Investigación Agropecuaria (INIA), Programa Pasturas y Forrajes, Estación Experimental INIA Tacuarembó, Ruta 5 km 386, Tacuarembó, Uruguay; email:fcazzuli@inia.org.uy -- |
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Contenido : |
It is unclear to what extent and on which variables does supplementing beef cattle on native grasslands affect sward structure, specifically on the dynamics of its grazing horizons. Three hypotheses were tested: (i) during a grazing down process under similar forage allowance, supplemented animals take longer to finish each grazing stratum, than their unsupplemented counterparts, (ii) in both cases, the upper stratum will be heavily depleted before the subsequent strata are grazed, (iii) some species and/or forage fractions are consumed faster than others, regardless of the animals being supplemented (corn dried distillers grains with solubles, DDGS, at 0.7% of their body weight, BW, on a dry matter, DM, basis) or not. © 2023 John Wiley & Sons Ltd. |
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Palabras claves : |
Beef cattle; Grazing down process; Grazing horizons; Protein-energy supplementation; Stockpiled native grasslands. |
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Asunto categoría : |
L02 Alimentación animal |
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Marc : |
LEADER 02168naa a2200349 a 4500
001 1064045
005 2024-01-26
008 2023 bl uuuu u00u1 u #d
022 $a0142-5242
024 7 $a10.1111/gfs.12608$2DOI
100 1 $aCAZZULI, F.
245 $aThe defoliation dynamics of a stockpiled native grassland pasture follow similar patterns between supplemented and unsupplemented beef calves.$h[electronic resource]
260 $c2023
500 $aArticle history: Received 24 November 2022; Revised 14 March 2023; Accepted 15 March 2023. -- Correspondence author: Cazzuli, F.; Instituto Nacional de Investigación Agropecuaria (INIA), Programa Pasturas y Forrajes, Estación Experimental INIA Tacuarembó, Ruta 5 km 386, Tacuarembó, Uruguay; email:fcazzuli@inia.org.uy --
520 $aIt is unclear to what extent and on which variables does supplementing beef cattle on native grasslands affect sward structure, specifically on the dynamics of its grazing horizons. Three hypotheses were tested: (i) during a grazing down process under similar forage allowance, supplemented animals take longer to finish each grazing stratum, than their unsupplemented counterparts, (ii) in both cases, the upper stratum will be heavily depleted before the subsequent strata are grazed, (iii) some species and/or forage fractions are consumed faster than others, regardless of the animals being supplemented (corn dried distillers grains with solubles, DDGS, at 0.7% of their body weight, BW, on a dry matter, DM, basis) or not. © 2023 John Wiley & Sons Ltd.
653 $aBeef cattle
653 $aGrazing down process
653 $aGrazing horizons
653 $aProtein-energy supplementation
653 $aStockpiled native grasslands
700 1 $aBREMM, C.
700 1 $aJAURENA, M.
700 1 $aPOPPI, D.
700 1 $aDURANTE, M.
700 1 $aBENVENUTTI, M.A.
700 1 $aSAVIAN, J.V.
700 1 $aDEVINCENZI, T.
700 1 $aROVIRA, P.J.
700 1 $aLAGOMARSINO, X.
700 1 $aHIRIGOYEN, A.
700 1 $aLATTANZI, F.
773 $tGrass and Forage Science, 2023, Vol.78, Issue 2, pages 306-316. doi: https://doi.org/10.1111/gfs.12608
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